AM
Alexey A. Makarov
Deuterium Exchange MeasurementHDXPeptidesHydrogen Deuterium Exchange-Mass SpectrometrySECBiological ProductsChromatography, GelChromatography, Reverse-PhaseDrug DiscoveryFrictional heating
On Valency
published · living versionsW_f6w2p2ta·v1 · currentpublished
Can the analyte-triggered asymmetric autocatalytic Soai reaction serve as a universal analytical tool for measuring enantiopurity and assigning absolute configuration?
with Christopher J. Welch, Kerstin Zawatzky, Satoshi Fujiwara, Arimasa Matsumoto +1
1 version
Preprints & journals
51 papers in the corpus · 2008–2026An Ultra-High Throughput Hydrogen-Deuterium Exchange Workflow Using Acoustic Ejection Mass Spectrometry for Studying Peptide Solution Structural Conformation.42366520 · Abucayon, Erwin G, Liu, Chang, Makarov, Alexey A et al.2026 · 0 citationsAnalytical chemistry. 2026;98(27):20066-20074
Direct Injection Mass Spectrometry and iFishMass for the High-Throughput Analysis of Antibody Modifications.41536291 · Aguilan, Jennifer, Madrid-Aliste, Carlos, Zandkarimi, Fereshteh et al.2026 · 1 citationACS pharmacology & translational science. 2026;9(1):165-176
Overexpression of Matriptase-1 Inhibitor HAI-1 as an Effective Strategy to Reduce Proteolytic Clipping in Chinese Hamster Ovary Cells.41140002 · Zhang, Qinghao, Aapjeet, Fnu, Chen, Joyce et al.2025 · 1 citationBiotechnology journal. 2025;20(10):e70141
Accelerating biotherapeutics discovery: Blueprint for a universal LC-MALDI-MS offline approach.40669996 · Bayne, Elizabeth F, Gao, Xiong-Zhuo, Jiang, Yuan et al.2025 · 1 citationAnalytica chimica acta. 2025;1368:344312
Strategies for automated affinity purification-resin screening for non-traditional biopharmaceuticals in the discovery space.39705847 · Cave, Jordan R, Makarov, Alexey A, Pirrone, Gregory F2025 · 0 citationsJournal of pharmaceutical and biomedical analysis. 2025;255:116637
Strategy for improving circular dichroism spectra deconvolution accuracy for macrocyclic peptides in drug discovery.39298840 · Guo, Wen, Makarov, Alexey A, Buevich, Alexei V et al.2024 · 8 citationsJournal of pharmaceutical and biomedical analysis. 2025;252:116476
Automated High-Throughput Matrix Assisted Laser Desorption Ionization Mass Spectrometry Methodology for Formulation Assessment of Polyethylene-Glycol-Conjugated Cytokine Proteins.37478972 · Pirrone, Gregory F, Munsell, Erik V, Ferguson, Heidi M et al.2026 · 1 citationJournal of pharmaceutical sciences. 2023;112(11):2778-2782
High-throughput infrared spectroscopy for quantification of peptides in drug discovery.37001275 · Hendrick, Nathaniel, Fraser, Douglas, Bennett, Raffeal et al.2023 · 4 citationsJournal of pharmaceutical and biomedical analysis. 2023;229:115350
Structural remodeling of SARS-CoV-2 spike protein glycans reveals the regulatory roles in receptor-binding affinity.36370046 · Hsu, Yen-Pang, Frank, Martin, Mukherjee, Debopreeti et al.2023 · 16 citationsGlycobiology. 2023;33(2):126-137
Solid-to-Liposome Conformational Transition of Phosphatidylcholine and Phosphatidylserine Probed by Atomic Force Microscopy, Infrared Spectroscopy, and Density Functional Theory Calculations.36107722 · Dou, Tianyi, Zens, Clara, Schröder, Katrin et al.2022 · 11 citationsAnalytical chemistry. 2022;94(38):13243-13249
Rapid label-free cell-based Approach Membrane Permeability Assay using MALDI-hydrogen-deuterium exchange mass spectrometry for peptides.36038238 · Makarov, Alexey A, Jiang, Yuan, Sondey, Christopher et al.2022 · 12 citationsAnalytica chimica acta. 2022;1225:340234
A chemoenzymatic strategy for site-selective functionalization of native peptides and proteins.35709255 · Fryszkowska, Anna, An, Chihui, Alvizo, Oscar et al.2022 · 55 citationsScience (New York, N.Y.). 2022;376(6599):1321-1327
Trapping-Enrichment Multi-dimensional Liquid Chromatography with On-Line Deuterated Solvent Exchange for Streamlined Structure Elucidation at the Microgram Scale.35139257 · Ahmad, Imad A Haidar, Losacco, Gioacchino Luca, Shchurik, Vladimir et al.2022 · 21 citationsAngewandte Chemie (International ed. in English). 2022;61(21):e202117655
Rapid antibody conformational screening by matrix-assisted laser desorption/ionization hydrogen-deuterium exchange mass spectrometry.35108448 · Mukherjee, Debopreeti, Trigo-Mouriño, Pablo, Jiang, Yuan et al.2022 · 5 citationsJournal of separation science. 2022;45(12):2055-2063
Pd Reaction Intermediates in Suzuki-Miyaura Cross-Coupling Characterized by Mass Spectrometry.35112808 · Chen, Xingshuo, Wei, Zhenwei, Huang, Kai-Hung et al.2025 · 7 citationsChemPlusChem. 2022;87(3):e202100545
Interrogation of solution conformation of complex macrocyclic peptides utilizing a combined SEC-HDX-MS, circular dichroism, and NMR workflow.34927633 · Adpressa, Donovon A, Reibarkh, Mikhail, Jiang, Yuan et al.2022 · 10 citationsThe Analyst. 2022;147(2):325-332
Enantioselective UHPLC Screening Combined with In Silico Modeling for Streamlined Development of Ultrafast Enantiopurity Assays.34931812 · Losacco, Gioacchino Luca, Wang, Heather, Haidar Ahmad, Imad A et al.2022 · 50 citationsAnalytical chemistry. 2022;94(3):1804-1812
Development of ProTx-II Analogues as Highly Selective Peptide Blockers of Nav1.7 for the Treatment of Pain.34931831 · Adams, Gregory L, Pall, Parul S, Grauer, Steven M et al.2022 · 19 citationsJournal of medicinal chemistry. 2022;65(1):485-496
In silico method development for the reversed-phase liquid chromatography separation of proteins using chaotropic mobile phase modifiers.33845343 · Haidar Ahmad, Imad A, Bennett, Raffeal, Makey, Devin et al.2025 · 17 citationsJournal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2021;1173:122587
Ultra-high-throughput SPE-MALDI workflow: Blueprint for efficient purification and screening of peptide libraries.33280687 · Bennett, Raffeal, Pirrone, Gregory F, Nowak, Timothy et al.2021 · 18 citationsAnalytica chimica acta. 2021;1142:10-18
Use of Raman spectroscopy and size-exclusion chromatography coupled with HDX-MS spectroscopy for studying conformational changes of small proteins in solution.32574996 · Wang, Rui, Mangion, Ian, Makarov, Alexey A et al.2021 · 12 citationsJournal of pharmaceutical and biomedical analysis. 2020;189:113399
Comprehensive online multicolumn two-dimensional liquid chromatography-diode array detection-mass spectrometry workflow as a framework for chromatographic screening and analysis of new drug substances.32128644 · Pickens, Chad J, Haidar Ahmad, Imad A, Makarov, Alexey A et al.2020 · 19 citationsAnalytical and bioanalytical chemistry. 2020;412(11):2655-2663
Combination of HDX-MS and in silico modeling to study enzymatic reactivity and stereo-selectivity at different solvent conditions.32036298 · Makarov, Alexey A, Iacob, Roxana E, Pirrone, Gregory F et al.2020 · 7 citationsJournal of pharmaceutical and biomedical analysis. 2020;182:113141
Chaotropic Effects in Sub/Supercritical Fluid Chromatography via Ammonium Hydroxide in Water-Rich Modifiers: Enabling Separation of Peptides and Highly Polar Pharmaceuticals at the Preparative Scale.31549812 · Liu, Jinchu, Makarov, Alexey A, Bennett, Raffeal et al.2020 · 82 citationsAnalytical chemistry. 2019;91(21):13907-13915
Liposome Artificial Membrane Permeability Assay by MALDI-hydrogen-deuterium exchange mass spectrometry for peptides and small proteins.31986267 · Makarov, Alexey A, Pirrone, Gregory F, Shchurik, Vladimir et al.2020 · 24 citationsAnalytica chimica acta. 2020;1099:111-118
The Emergence of Universal Chromatographic Methods in the Research and Development of New Drug Substances.31198042 · Regalado, Erik L, Haidar Ahmad, Imad A, Bennett, Raffeal et al.2020 · 67 citationsAccounts of chemical research. 2019;52(7):1990-2002
Multi-column ultra-high performance liquid chromatography screening with chaotropic agents and computer-assisted separation modeling enables process development of new drug substances.30830135 · Haidar Ahmad, Imad A, Chen, Wenyong, Halsey, Holst M et al.2019 · 38 citationsThe Analyst. 2019;144(9):2872-2880
Evaluation of global conformational changes in peptides and proteins following purification by supercritical fluid chromatography.30785083 · Schiavone, Nicole M, Bennett, Raffeal, Hicks, Michael B et al.2019 · 47 citationsJournal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2019;1110-1111:94-100
Use of MALDI-MS with solid-state hydrogen deuterium exchange for semi-automated assessment of peptide and protein physical stability in lyophilized solids.30712581 · Kabaria, Sneha R, Mangion, Ian, Makarov, Alexey A et al.2019 · 19 citationsAnalytica chimica acta. 2019;1054:114-121
Generic anion-exchange chromatography method for analytical and preparative separation of nucleotides in the development and manufacture of drug substances.30591245 · Tsay, Fuh-Rong, Haidar Ahmad, Imad A, Henderson, Derek et al.2019 · 33 citationsJournal of chromatography. A. 2019;1587:129-135
pKa measurements for the SAMPL6 prediction challenge for a set of kinase inhibitor-like fragments.30406372 · Işık, Mehtap, Levorse, Dorothy, Rustenburg, Ariën S et al.2019 · 48 citationsJournal of computer-aided molecular design. 2018;32(10):1117-1138
Combination of circular dichroism spectroscopy and size-exclusion chromatography coupled with HDX-MS for studying global conformational structures of peptides in solution.30609519 · Schiavone, Nicole M, Pirrone, Gregory F, Guetschow, Erik D et al.2019 · 13 citationsTalanta. 2019;194:177-182
Visualizing and studying frictional heating effects in reversed-phase liquid chromatography using infrared thermal imaging.29605126 · Makarov, Alexey A, Mann, Benjamin F, Regalado, Erik L et al.2018 · 18 citationsAnalytica chimica acta. 2018;1018:1-6
Supercritical fluid chromatography-photodiode array detection-electrospray ionization mass spectrometry as a framework for impurity fate mapping in the development and manufacture of drug substances.29477066 · Pirrone, Gregory F, Mathew, Rose M, Makarov, Alexey A et al.2018 · 35 citationsJournal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2018;1080:42-49
Macrocyclic glycopeptide chiral selectors bonded to core-shell particles enables enantiopurity analysis of the entire verubecestat synthetic route.29397980 · Barhate, Chandan L, Lopez, Diego A, Makarov, Alexey A et al.2018 · 55 citationsJournal of chromatography. A. 2018;1539:87-92
Use of MALDI-MS Combined with Differential Hydrogen-Deuterium Exchange for Semiautomated Protein Global Conformational Screening.28727449 · Pirrone, Gregory F, Wang, Heather, Canfield, Nicole et al.2019 · 28 citationsAnalytical chemistry. 2017;89(16):8351-8357
Semi-automated screen for global protein conformational changes in solution by ion mobility spectrometry-massspectrometry combined with size-exclusion chromatography and differential hydrogen-deuterium exchange.28347518 · Pierson, Nicholas A, Makarov, Alexey A, Strulson, Christopher A et al.2017 · 13 citationsJournal of chromatography. A. 2017;1496:51-57
Effects of pressure and frictional heating on protein separation using monolithic columns in reversed-phase chromatography.28193469 · Mann, Benjamin F, Makarov, Alexey A, Wang, Heather et al.2017 · 7 citationsJournal of chromatography. A. 2017;1489:58-64
Ultrafast Chiral Chromatography as the Second Dimension in Two-Dimensional Liquid Chromatography Experiments.28192943 · Barhate, Chandan L, Regalado, Erik L, Contrella, Nathan D et al.2019 · 120 citationsAnalytical chemistry. 2017;89(6):3545-3553
Unusual reversal of enantioselectivity in the asymmetric autocatalysis of pyrimidyl alkanol triggered by chiral aromatic alkanols and amines.27942665 · Matusmoto, Arimasa, Fujiwara, Satoshi, Hiyoshi, Yui et al.2018 · 27 citationsOrganic & biomolecular chemistry. 2017;15(3):555-558
Ultrafast chiral separations for high throughput enantiopurity analysis.27872920 · Barhate, Chandan L, Joyce, Leo A, Makarov, Alexey A et al.2017 · 132 citationsChemical communications (Cambridge, England). 2017;53(3):509-512
Can the analyte-triggered asymmetric autocatalytic Soai reaction serve as a universal analytical tool for measuring enantiopurity and assigning absolute configuration?27714244 · Welch, Christopher J, Zawatzky, Kerstin, Makarov, Alexey A et al.2018 · 11 citationsOrganic & biomolecular chemistry. 2016;15(1):96-101
Use of hydrostatic pressure for modulation of protein chemical modification and enzymatic selectivity.27088756 · Makarov, Alexey A, Helmy, Roy, Joyce, Leo et al.2018 · 5 citationsOrganic & biomolecular chemistry. 2016;14(19):4448-55
Combining size-exclusion chromatography with differential hydrogen-deuterium exchange to study protein conformational changes.26763301 · Makarov, Alexey A, Helmy, Roy2016 · 20 citationsJournal of chromatography. A. 2016;1431:224-230
Use of pressure in reversed-phase liquid chromatography to study protein conformational changes by differential deuterium exchange.25620238 · Makarov, Alexey A, Schafer, Wes A, Helmy, Roy2015 · 15 citationsAnalytical chemistry. 2015;87(4):2396-402
Search for improved fluorinated stationary phases for separation of fluorine-containing pharmaceuticals from their desfluoro analogs.25595532 · Regalado, Erik L, Makarov, Alexey A, McClain, Ray et al.2015 · 37 citationsJournal of chromatography. A. 2015;1380:45-54
Effect of pressure on the chromatographic separation of enantiomers under reversed-phase conditions.24928149 · Makarov, Alexey A, Regalado, Erik L, Welch, Christopher J et al.2014 · 7 citationsJournal of chromatography. A. 2014;1352:87-92
Chromatographic resolution of closely related species in pharmaceutical chemistry: dehalogenation impurities and mixtures of halogen isomers.24359254 · Regalado, Erik L, Zhuang, Ping, Chen, Yadan et al.2014 · 66 citationsAnalytical chemistry. 2014;86(1):805-13
Effect of pressure on secondary structure of proteins under ultra high pressure liquid chromatographic conditions.24140255 · Makarov, Alexey, LoBrutto, Rosario, Karpinski, Paul2014 · 15 citationsJournal of chromatography. A. 2013;1318:112-21
Discovery of a stable molecular complex of an API with HCl: a long journey to a conventional salt.18240276 · Peresypkin, Andrey, Variankaval, Narayan, Ferlita, Russell et al.2008 · 11 citationsJournal of pharmaceutical sciences. 2008;97(9):3721-6
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